Production Engineering - Medical Equipment Division

PEMED HOME 6035 E. 38th Avenue, Denver, Colorado 80207 USA (303) 393-7800 FAX (303) 393-1482

Laboratory Synthesizers

DNA synthesizer - $2,750

List: $26,700. MedCon: VG. Applied Biosystems ABI model 391 PCR-Mate EP DNA Synthesizer.This is a current model of this instrument sold and services by Perkin-Elmer. Used to produce synthetic nucleic acids for most applications: PCR, sequencing, antisense, gene construction, hybridization, and the like.

Features:

• compact bench-top size
• 2-hour primer synthesis
• precise liquid delivery system
• full programmability of cycles, functions and reagent pathways
• 4 scales: 40 nmol, 0.2-, 1.0- and 10-µmol
• Simple operation: load reagents, key in the sequence, and press "start."
• Five phosphoramidite bottle positions.
• Straightforward entry of mixed base sites and documented competitive coupling produce reliable degenerate primers.
• programmable consumption alarms
• built-in bottle change procedure

The ABI 391 can deliver a primer-length oligonucleotide in under two hours, and a PCR primer set in less than half a day. That makes it possible to go from primer design to PCR results in a normal working day. Moreover, robust chemistry and programmable cycles make it equally adept at synthesis of longer oligonucleotides.

This unit will hook directly to a McIntosh. This Hypercard® program lets you import, edit and store sequences on the computer, then download them to the ABI 391 without additional keystrokes.

Specifications:

• DNA (cyanoethyl phosphoramidite): 40 nmol, 0.2 µmol, 1.0 µmol, 10 µmol
• Reagent delivery: argon gas pressure
• Valve design: zero-dead-volume solenoid valves in patented valve blocks
• Solvent/reagent bottles: 5
• Display: 2-line, 40-character LCD
• Programmability: cycle, procedure, function
• Step time quantization: 1 second
• Chemical authenticity: >99.8%
• Coupling efficiency: >98%
• Cycle time: 5.6 minutes
• Waste per cycle: <7 ml
• Reservoir capacity: 12 ml, 200 ml, 450 ml, and 4 L external bottles
• Mixed-base synthesis capability: +/- 10% of each base in the mixture
• Power: 100-240 VAC 50/60 Hz
• parallel printer port
• serial RS232 computer port
• dimensions: 21" wide, 16" high, 15" deep
• shipping weight: 75 pounds
• connection in back for argon gas (75 psi max)
• connections in back for trityl output, delivery and pressure

Here are some current technical papers available from applied biosystems web site (http://www.pebio.com/ab/techsupp/doclib.html)

• Fast, Economical Oligonucleotide Synthesis At The 0.2µmol Scale
• Synthesis and Purification of Fluorescently Labeled Oligonucleotides Using Dye Phosphoramidites
• Chemical Storage Conditions
• FastPhoramidite Reagents for DNA Synthesis with Rapid Deprotection at All Scales
• Phosphalink Amidite for Phosphorylation of Oligonucleotides
• Biotin Labeling of Oligonucleotides on a DNA/RNA Synthesizer

Features:

Overall dimensions of the unit:

• width: 20.5 in.
• depth: 16
• height: 16.5

Shipping weight is about 95 pounds.

Gel image digitizer - $975

List: $27,800. MedCon: F. Genomyx SC gel scanner and image digitizer, model FL100.100-120/220-240 V, 50-60 Hz.

Powers up, but light source did not come on. Xenon bulb may need to be replaced. Computer workstation is not included.

This unit is essentially a scanning plate densitometer, running DNA electrophoresis plates.

Features:

• XLS-300 xenon light source
• xy transport mechanism for scanner
• 9- and 15-pin I/O connectors on back

Overall dimensions of the unit:

• width: 31.5 in.
• depth: 19
• height: 30.5

Shipping weight is about 180 pounds.

DNA sequencing electrophoresis system - $2,750

List: 92,900. MedCon: VG. Genomyx LR DNA programmable high resolution DNA sequencer, model GX100. 120 V, 60 Hz.

Features:

• supports for holding electrophoresis plates and anode and cathode connectors
• keypad to enter programs for voltage, time, Watts and temp
• cooling fan
• 9-pin I/O

Overall dimensions of the unit:

• width: 31.25 in.
• depth: 20
• height: 30.5

Shipping weight is about 200 pounds.

PCR Sequence Detector - $975

List: 8,230. MedCon: VG. Perkin-Elmer/Applied Biosystems Prism 7200 sequence detector, model B8010050. 120/230 V, 50/60 Hz.

Uses a 96 well platform to quantify fluorescent labeled nucleotides incorporated into DNA in the polymerase chain reaction. Back of unit has serial, parallel and 5-pin barcode ports.

Overall dimensions of the unit:

• width: 13.5 in.
• depth: 18.25
• height: 14

Shipping weight is about 50 pounds.

Organic molecule synthesizer - $9,750

List: $135,700. MedCon: VG. Argonaut Technologies Nautilus 2400 synthesizer. 100-120 V. This instrument is for prototyping and optimizing the reaction conditions for organic synthesis in applications such as drug discovery. Three rocking racks contain 8 reaction chambers each (see photo), allowing for 24 variations of a reaction to be processed simultaneously.

For example, 24 minor variations of a drug manufacturing reaction can be done simultaneously. Then, the concentrations of the 24 reactions can be measured, providing the two or three variations that seem to be the most successful. This greatly speeds up the development process.

For example, individualized variations can be made in vessel size, reagent concentrations and reaction time and temperature. This reaction module can be interfaced with an autosampler and a reagent delivery chamber (not included).

chamber open
rack controls
vessel racks
vessel racks (detail)
manufacturer's plate

Overall dimensions:

• width: 45 in.
• depth: 26
• height: 19

Shipping weight is about 300 pounds.

Peptide synthesizer - $2,750

List: $47,300. MedCon: VG. No Software. Applied Biosystems model 430A peptide synthesizer. 100-120/200-240 V, 50/60 Hz.

Uses tBOC or FMOC protecting group chemistry for sequential peptide synthesis on column-bound resin. Computer and printer ports on back of instrument.

Overall dimensions of the unit:

• width: 31 in.
• depth: 21.5
• height: 61

Shipping weight is about 300 pounds.

DNA synthesizer - $2,175

List: $16,750. MedCon: VG. Applied Biosystems model 380B DNA synthesizer. 110 V, 60 Hz. Synthesis of a DNA chain is accomplished by addition of phoshoramidite derivatives of deoxynucleosides to an initial nucleoside covalently attached to a column support. All steps of the chemical delivery system are automated. The built-in monitor is a touch screen by which the user accesses programs such as the DNA sequence editor, the cycle editor, and the system self-test.

User's manual, software and miscellaneous supplies included.

Overall dimensions of the unit:

• width: 31 in.
• depth: 20
• height: 27

Shipping weight is about 240 pounds.

Peptide sequencer - $1,450

List: $55,200. MedCon: G. LKB Biochrom model 4020 Solid-Phase Peptide Sequencer. Programmable (through a program card) operation, bench top design of device.

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Production Engineering - Medical Equipment Division

6035 E. 38th Avenue, Denver, Colorado 80207 USA (303) 393-7800 FAX (303) 393-1482